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. 2014 May 16;9(5):e97199. doi: 10.1371/journal.pone.0097199

Figure 4. Representative images of individual embryos analyzed by allele-specific PCR for Oct4 and Nanog mRNA.

Figure 4

All of the embryos subjected to Oct4 (A) and Nanog (B) analysis were previously found positive for β-actin mRNA. Gel pictures show 7 embryos analyzed for Oct4 and 7 embryos analyzed for Nanog. (A) Oct4 transcripts from 3 Aph+ embryos are only maternal (C3H/HeN amplicon, single band uncut), whereas Oct4 transcript from 4 Aph− embryos are either maternal (no reprogramming) or somatic (C57Bl/6J reprogrammed, cut) or paternal (C57Bl/6J sperm, cut). (B) Aph+ embryos have no Nanog transcript whereas Aph− embryos have Nanog transcript from either the C3H/HeN or the C57Bl/6J allele.