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. 2014 May 16;9(5):e97199. doi: 10.1371/journal.pone.0097199

Figure 5. Results of individual embryos analyzed by allele-specific PCR for Oct4 and Nanog mRNA.

Figure 5

(A) Pooled frequencies of Oct4 and (B) Nanog mRNA expression in SCNT or ICSI embryos after treatment with Aph or CB from 6 hpa. (C) Frequencies of Oct4 mRNA expression in SCNT embryos after treatment with Aph or CB from 6 or 24 hpa. (D) Frequencies of Oct4 mRNA expression in ICSI embryos after treatment with Aph or CB from 6 or 24 hpa. (E) Frequencies of Nanog mRNA expression in SCNT embryos after treatment with Aph or CB from 6 or 24 hpa. (F) Frequencies of Nanog mRNA expression in ICSI embryos after treatment with Aph or CB from 6 or 24 hpa. All of the abovementioned embryos tested positive for β-actin mRNA. Statistical significance was calculated using chi-square test with p≤0.05.