. 2014 May 16;9(5):e97199. doi: 10.1371/journal.pone.0097199

Table 2. OCT4-GFP expression in SCNT and ICSI embryos (C3HxOG2).

	GFP⁺/total (%)
Groups	Aph⁻	Aph⁺	CB⁺
96 hpa SCNT (treated at 6 hpa)	59/65 (90.8)^a	0/52 (0)^b	28/75 (37.3)^b
96 hpa SCNT (treated at 24 hpa)	59/65 (90.8)^a	0/211 (0)^b	8/13 (61.5)^b
96 hpa ICSI (treated at 6 hpa)	36/36 (100)^a	0/27 (0)^b	45/54 (83.3)^b
96 hpa ICSI (treated at 24 hpa)	36/36 (100)^a	n.p.	28/28 (100)^a

To test the requirement of first round DNA replication and cell division for reprogramming, pronuclear oocytes at 6 hours post activation (hpa) were cultured in 2 µg/mL aphidicolin (aph) or in 5 µg/mL cytochalasin B (CB) until 96 hpa. In parallel, pronuclear oocytes were allowed to cleave and reach the blastocyst stage. To test the requirement of second round DNA replication and cell division for reprogramming, 2-cell embryos at 24 hpa were cultured in 2 µg/mL aph or in 5 µg/mL CB until 96 hpa and were compared with time-matched blastocyst-stage embryos. For each treatment and type of embryo, results are given as frequencies of embryos expressing GFP/embryos imaged. These frequencies, given as percent, were analyzed using chi-square test and applying the Bonferroni correction for multiple comparisons.

^a,b

: different superscripts within same row indicate significant difference (chi-square test).

n.p.: not performed.