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. 2014 May 16;9(5):e97574. doi: 10.1371/journal.pone.0097574

Figure 4. Binding of the RNA SELEX pools to gHA1.

Figure 4

The gHA1 protein charged in Ni-NTA spin trap columns was eluted with buffer solution containing the initial RNA pool, the 5th, 9th, and 12th round RNA pools, or no RNA (negative control, NC). Elution with imidazole buffer was collected and subjected to RT-RCR. Amplified cDNAs indicating the gHA1-bound RNAs were stained with ethidium bromide on 1.5% agarose gel after electrophoresis and were quantified. Relative amounts of the gHA1-bound RNAs are shown in the graph.