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. 2014 May 16;9(5):e97694. doi: 10.1371/journal.pone.0097694

Figure 3. Glucose stimulation induces up-regulation of both c-Abl and insulin expression.

Figure 3

A . NIT-1 cells were cultured in low glucose DMEM medium with or without glucose 16 mM for 6 hrs. Thereafter, the cells were examined for c-Abl and insulin gene expression using real-time RT-PCR. Student t test was performed. The results shown were from a representative of 3 independent experiments. *: p>0.05, **:p<0.001. B. NIT-1 cells were cultured in low glucose DMEM medium with or without glucose 16 mM for 24 hrs. Then, the cells were cytospun onto slides and stained with anti-c-Abl antibodies and Dappi, and visualized by a fluorescent microscope (Zeiss Axioskop). A representative image of three slides in each group is shown.