Figure 3. Effect of CRF on TLR4 and interleukins during differentiation of 3T3L1.
Pre-adipocytes were cultured in differentiating media supplemented with CRF at 10−8M, pre-treated with antalarmin at 10−6 M and the production of IL-6 and CXCL1 was measured by ELISA (Panels A, B). Pre-adipocytes were cultured in differentiating media supplemented with CRF at 10−8 M plus/minus LPS (10 ng/ml) and the expression of TLR4 was measured by RT-PCR (Panel C) and the production of IL-6 and CXCL1 (Panels D, E) was measured by ELISA. Data are expressed as percentage change compared with control values (mean±SE, n = 10 of five independent experiments). *p<0.05, **p<0.01, ***p<0.001 depict the statistical significant difference from cells exposed only to vehicles, while #p<0.05 depicts the statistical significant difference from cells exposed to LPS alone.