Figure 4. UCN2 affects the production of interleukins and the expression of TLR4 by pre-adipocytes.
Pre-adipocytes were exposed to UCN2 at 10−8M and the production of interleukins was measured by ELISA (Panel A), the TLR4 mRNA levels were measured by RT-PCR (Panel B) and TLR4 protein levels were measured by FACS analysis (Panel C) at several time intervals. Data are expressed as mean±SE, n = 6 of three independent experiments. *p<0.05 depicts the statistical significant difference from cells exposed only to vehicles.