Figure 9.1.

Immunofluorescent screening of α2A&C AR trafficking motifs. (A) Surface and total staining of WT HA-α2A&C ARs, co-stained with the ER marker calreticulin, demonstrate predominant intracellular (ER) localization of α2C, compared to α2A, ARs. α2A ARs are primarily found at the plasma membrane. (B) Total cellular staining of α2A/C AR chimeras demonstrates that the extracellular amino terminus of α2A and α2C ARs is a determinant of their relative expression patterns. (C) Removal of an α2C AR amino-terminal hydrophobic region (ALAAALAAAAA =α2CΔ5-15 AR) enhances cell surface expression, as assessed by total cellular staining, suggesting a possible role for this domain in α2C AR membrane expression. Adapted from Angelotti et al. (2010).