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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Hum Genet. 2013 Dec 22;133(6):743–753. doi: 10.1007/s00439-013-1407-z

Fig. 2.

Fig. 2

Development of Dp(16)3Yey/+ mice based on recombinase-mediated chromosome engineering. (a) Schematic representation of the strategy used to generate Dp(16)3Yey and Df(16)3Yey. B, BamHI; H, HindIII; 5’, 5’HPRT fragment; 3’, 3’HPRT fragment; N, Neomycin-resistance gene; P, puromycin-resistance gene; Ty, Tyrosinase transgene; Ag, Agouti transgene; arrowhead, loxP site. (b) Southern blot anaylsis of ES cell DNA digested with BamHI (lanes 1, 3 and 4) or HindIII (lane 2) using Probe A (lanes 1 and 3), Probe B (lane 2) or Probe C (lane 4), respectively. (c) FISH analysis of chromosomes of the engineered ES cells. (i) Schematic representation of the genomic locations of BAC probes for FISH analysis. (ii) FISH analysis of metaphase nuclei prepared from the ES cells carrying Dp(16)3Yey/Df(16)3Yey. (iii) FISH analysis of interphase chromosomes prepared from the ES cells carrying Dp(16)3Yey/Df(16)3Yey.