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. Author manuscript; available in PMC: 2014 May 17.
Published in final edited form as: J Allergy Clin Immunol. 2008 Jun 12;122(3):521–528. doi: 10.1016/j.jaci.2008.05.002

FIG 2.

FIG 2

Enhanced SP-D expression in the airways is directly stimulated by IL-13. A, BALB/c mice were sensitized and challenged with Aspergillus fumigatus (Af) and studied at the indicated time points. B, Constitutive TNF-α expression was targeted into lung type II alveolar epithelial cells by using an SP-C promoter construct. C, Recombinant mouse IL-13 (1.5 μg) or BSA was administered intratracheally to sensitized mice, and BAL fluid SP-D levels were studied 24 hours later. D, Isolated rat type II alveolar epithelial cells were cultured with IL-13 for 4 days. E, IL-13–deficient mice were sensitized and challenged with the allergen and studied 24 hours later. IL-13 protein in BAL fluid (Fig 2, A) was assessed by means of ELISA. IL-13 mRNA (Fig 2, A and B) in the lung was studied by means of real-time PCR. SP-D protein content was analyzed by using Western blotting (Fig 2, C–E). Results are presented as means ± SEMs (n ≥ 6). *P < .05. N, Naive mice; Wt, wild-type, TNFα-tg, TNF-α–transgenic mice.