Fig. 5.

Intratumoral treatment with LTX-315 induces an inflammatory response. Following a single i.t. injection of sterile 0.9 % NaCl (control), or with 1 mg LTX-315, tumor tissue (a) and plasma (b) were harvested 4, 24, 48 and 120 h post-injection and analyzed for mRNA expression of several different cytokines using RT qPCR (tumor tissue) or for cytokine content using ELISA (plasma). Data from three animals are presented for each time point as mean ± SEM. Normal tumor expression (control) was defined as 1 (normal expression = normal/normal), while treated tumor expression was defined as treated tumor = treated tumor/normal (a). Statistical analysis was performed using a Tukey′s multiple comparison test (p < 0.05). Data from animals treated with either LTX-315 (n = 4) and vehicle (n = 2) are presented as the pg/ml content of IL6 in pooled plasma samples (b)