Fig. 3.

Post-proteomic validation of the selected proteins using individual patients from control and VaD groups by WB analysis. Equal amount of protein was loaded as measured by the 2D Quant kit. A) Representative immunoblots showing the protein levels in B21 area of all twenty patients (n = 10 per group). Details of the patients can be found in the Supplemental Table 1. ACTB was used as a loading control. B) Bar chart of densitometric analysis for comparing the protein expression levels by the statistical analysis. SOD1 and NCAM were significantly increased whereas ATP5A was reduced significantly in the VaD brain. Trends were observed for SYNPO, HSPA4, VADC1, ferritin, PEA15 and ICAM5 without reaching a statistical significance. Data was presented as mean ± SEM (n = 10), where *p < 0.05, significantly different from control using independent-sample t-test.