Table 2.
Results of progeny testing of 41 cold-tolerant BC 2 F 2 individuals from four BC populations based on survival percentages (SPs) of their derived BC 2 F 2:6 progeny evaluated under conditions of 4°C for 7 d in a growth chamber
| Pop. 1 | BC 2 F 2 selected ID | BC 2 F 2 verified ID | # of BC 2 F 2:6 ILs | Z -test 2 | SP ± S.D. (%) 3 |
Cold response index
4
|
|
|---|---|---|---|---|---|---|---|
| SH ± SD (%) | SDW ± SD (%) | ||||||
| ZH100 |
031 K85 |
A-IL1 |
1 |
|
30.0 ± n.a.** |
81.0 ± n.a. |
75.4 ± n.a. |
| |
031 K86 |
A-IL2 |
2 |
|
46.1 ± 25.6** |
80.3 ± 11.0 |
75.0 ± 16.0 |
| |
031 K89 |
A-IL3 |
5 |
√ |
23.7 ± 18.4* |
88.5 ± 23.4 |
88.5 ± 35.3 |
| |
031 K90 |
A-IL4 |
2 |
|
40.3 ± 0.2** |
100.7 ± 20.7 |
102.4 ± 10.6 |
| |
031 K92 |
A-IL5 |
5 |
√ |
41.4 ± 26.7** |
91.7 ± 10.2 |
101.9 ± 33.8 |
| |
031 K97 |
A-IL6 |
1 |
|
66.0 ± n.a.** |
75.9 ± n.a. |
59.4 ± n.a. |
| |
031 K96 |
- |
1 |
|
9.7 ± n.a. |
95.5 ± n.a. |
82.4 ± n.a. |
| |
031 K101 |
- |
1 |
|
10.0 ± n.a. |
70.9 ± n.a. |
82.5 ± n.a. |
| Bg300 |
031 K188 |
B-IL1 |
26 |
√ |
66.6 ± 30.2** |
87.5 ± 12.4 |
114.1 ± 30.8 |
| |
031 K187 |
B-IL2 |
10 |
|
23.8 ± 9.5* |
83.8 ± 9.4 |
113.6 ± 31.6 |
| |
031 K190 |
B-IL3 |
5 |
|
40.2 ± 4.0** |
82.5 ± 9.7 |
134.2 ± 30.6 |
| |
031 K195 |
B-IL4 |
11 |
√ |
20.7 ± 22.3* |
76.3 ± 7.0 |
97.4 ± 19.0 |
| |
031 K196 |
B-IL5 |
8 |
√ |
17.2 ± 22.2* |
72.1 ± 6.1 |
97.7 ± 20.7 |
| |
031 K189 |
B-IL6 |
2 |
|
41.5 ± 0.8** |
71.7 ± 7.9 |
90.9 ± 3.8 |
| |
031 K186 |
- |
1 |
|
0.0 ± n.a. |
78.0 ± n.a. |
82.1 ± n.a. |
| |
031 K191 |
- |
6 |
|
1.2 ± 1.8 |
86.1 ± 7.0 |
117.5 ± 35.4 |
| |
031 K192 |
- |
1 |
|
0.0 ± n.a. |
82.2 ± n.a. |
90.8 ± n.a. |
| |
031 K193 |
- |
5 |
|
17.2 ± 13.4 |
88.6 ± 15.1 |
117.5 ± 17.7 |
| |
031 K194 |
- |
6 |
|
0.0 ± 0.0 |
81.6 ± 12.2 |
98.5 ± 14.0 |
| Cis |
031 K155 |
C-IL1 |
7 |
√ |
30.2 ± 22.2** |
81.6 ± 16.9 |
88.3 ± 30.7 |
| |
031 K156 |
C-IL2 |
14 |
√ |
22.2 ± 23.6* |
73.5 ± 13.1 |
77.1 ± 17.5 |
| |
031 K158 |
C-IL3 |
11 |
√ |
46.5 ± 35.5** |
79.4 ± 8.6 |
74.1 ± 19.7 |
| |
031 K159 |
C-IL4 |
1 |
|
95.4 ± n.a.** |
90.2 ± n.a. |
106.0 ± n.a. |
| |
031 K160 |
C-IL5 |
5 |
|
82.2 ± 20.7** |
78.8 ± 7.7 |
86.5 ± 26.7 |
| |
031 K161 |
C-IL6 |
2 |
|
85.1 ± 0.3** |
89.9 ± 5.8 |
113.7 ± 27.3 |
| |
031 K162 |
C-IL7 |
3 |
|
66.0 ± 4.3** |
84.0 ± 4.3 |
79.0 ± 30.9 |
| |
031 K163 |
C-IL8 |
1 |
|
74.6 ± n.a.** |
76.3 ± n.a. |
59.2 ± n.a. |
| |
031 K165 |
C-IL9 |
2 |
|
79.2 ± 23.9** |
79.1 ± 20.8 |
80.4 ± 16.8 |
| |
031 K168 |
C-IL10 |
1 |
|
68.6 ± n.a.** |
67.3 ± n.a. |
73.8 ± n.a. |
| |
031 K170 |
C-IL11 |
1 |
|
78.2 ± n.a.** |
96.0 ± n.a. |
90.1 ± n.a. |
| |
031 K171 |
C-IL12 |
4 |
|
69.5 ± 16.3** |
76.5 ± 5.2 |
92.3 ± 17.0 |
| |
031 K172 |
C-IL13 |
1 |
|
53.3 ± n.a.** |
72.4 ± n.a. |
92.4 ± n.a. |
| |
031 K166 |
- |
1 |
|
14.3 ± n.a. |
91.1 ± n.a. |
55.9 ± n.a. |
| |
031 K157 |
- |
1 |
|
15.8 ± n.a. |
65.1 ± n.a. |
82.0 ± n.a. |
| MNTH |
031 K5 |
D-IL1 |
3 |
|
67.6 ± 28.2** |
85.7 ± 22.6 |
166.9 ± 55.7 |
| |
031 K12 |
D-IL2 |
2 |
|
52.8 ± 3.2** |
65.5 ± 2.7 |
89.7 ± 6.9 |
| |
031 K20 |
D-IL3 |
6 |
|
49.8 ± 12.9** |
70.9 ± 7.5 |
84.8 ± 3.4 |
| |
031 K21 |
D-IL4 |
2 |
|
46.6 ± 27.5** |
63.9 ± 15.1 |
88.3 ± 37.9 |
| |
031 K22 |
D-IL5 |
6 |
√ |
43.4 ± 16.0** |
78.3 ± 6.4 |
90.6 ± 19.2 |
| |
031 K8 |
- |
1 |
|
7.8 ± n.a. |
74.8 ± n.a. |
90.4 ± n.a. |
| |
031 K15 |
- |
3 |
|
11.9 ± 5.0 |
63.6 ± 21.2 |
41.0 ± 0.6 |
| C418 | 13.1 ± 7.1 | 73.9 ± n.a. | 77.6 ± n.a. | ||||
1ZH100, Cis, and MNTH represent Zihui100, Cisanggarung, and Manawthukha, respectively.
2“√” indicates that the two groups of BC2F2:6 ILs derived from the same BC2F2 individual had distinct CT phenotypes according to the two-sample proportion test.
3* and ** indicate that the SPs of BC2F2:6 ILs were significantly higher than the average SP of recurrent parent C418 at P < 0.05 and 0.01, respectively, according to the contrast test.
4SH and SDW are seedling height (in cm) and seedling dry weight (in g), respectively, under natural low-temperature stress.