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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Feb 24;1843(6):1196–1206. doi: 10.1016/j.bbamcr.2014.02.008

Table 2.

Primers and conditions used for ChIP analysis.

Gene Promoter Sequence Amplicon Length Cycling Conditions
Gria1 F: 5′ GGGAGGTGAAGGGGACAGTGGG 3′ 170 bp 94°: 30s, 57°: 30s,
72°: 45s; 2.5% DMSO
R: 5′ CCCAGGAGCCAGCTCGGAGT 3′
Gria2 F: 5′ ATCGAATGCGCAAGACTGGA 3′ 201 bp 94°: 30s, 60°: 30s,
72°: 45s; 2.5% DMSO
R: 5′ GAAAGTCACAGAGAGGGGCA 3′
Gria3 F: 5′ CAGAAATCGCTTTGGGGAACC 3′ 71 bp 94°: 30s, 60°: 30s,
72°: 45s; 2.5% DMSO
R: 5′ ACTTCTGTCTTCACACCAATCTG 3′
Gria4 F: 5′ AGCCTCCCTGAAGACCTGGGC 3′ 231 bp 94°: 30s, 60°: 30s,
72°: 45s; 2.5% DMSO
R: 5′ GGCTCCCTTGCGCTCCCTTG 3′
GM3 Synthase F: 5′ CACCTACTTCTCGGCTGGAG 3′ 198 bp 94°: 30s, 57°: 30s,
72°: 45s.
R: 5′ AATTCAGCCCCGGACAGT 3′
NT3 F: 5′ GAGCAAACTCCAAAATGCCAGG 3′ 219 bp 94°: 30s, 57°: 30s,
72°: 45s
R: 5′ AAAGTTGCGCCGGGCTATCTC 3′
β-Actin F: 5′ GCTCTTTTCCAGCCTTCCTT 3′ 187 bp 94°: 30s, 57°: 30s,
72°: 45s.
R: 5′ CGGATGTCAACGTCACACTT 3′