Table 4.
Sensitivity, lure specificity and Obsoletus specificity for assays using COI and ITS1 markers
| Marker | Tested effect | Lab | n | Sensitivity | n | Lure specificity | n | Obsoletus specificity | |
|---|---|---|---|---|---|---|---|---|---|
| COI |
Overall |
5 |
240 |
59.6 |
52 |
92.3 |
441 |
99.1 |
|
| |
Laboratory |
5 |
240 |
65.6 [48.4-81.2] |
52 |
92.3 [85.7-100.0] |
441 |
99.1 [97.6-100.0] |
|
| |
Species |
C. obsoletus |
5 |
60 |
61.7 |
- |
- |
107 |
99.1 |
| |
|
C. scoticus |
5 |
60 |
41.7 |
- |
- |
121 |
99.2 |
| |
|
C. dewulfi |
5 |
60 |
70.0 |
- |
- |
103 |
100.0 |
| |
|
C. chiopterus |
5 |
60 |
65.0 |
- |
- |
108 |
98.1 |
| |
Sample type |
Ground midge |
4 |
112 |
33.0 |
24 |
95.8 |
114 |
99.1 |
| |
|
Whole midge |
4 |
64 |
79.7 |
16 |
100.0 |
156 |
99.4 |
| |
|
DNA sample |
4 |
64 |
85.9 |
12 |
75.0 |
171 |
98.8 |
| |
DNA extraction method |
Crude lysate |
1 |
28 |
53.6 |
6 |
100.0 |
45 |
100.0 |
| |
|
Chelex method |
1 |
44 |
34.1 |
10 |
100.0 |
48 |
97.9 |
| |
|
Commercial kits |
3 |
168 |
67.9 [3.6-83.0] |
36 |
87.5 [83.3-100.0] |
345 |
100.0 [99.0-100.0] |
| ITS1 |
Overall |
7 |
288 |
76.4 |
64 |
81.2 |
795 |
94.7 |
|
| |
Laboratory |
7 |
288 |
79.7 [40.6-100.0] |
64 |
100.0 [42.9-100.0] |
795 |
95.0 [83.3-100.0] |
|
| |
Species |
C. obsoletus |
7 |
72 |
81.9 |
- |
- |
198 |
96.5 |
| |
|
C. scoticus |
7 |
72 |
80.6 |
- |
- |
203 |
86.2 |
| |
|
C. dewulfi |
7 |
72 |
79.2 |
- |
- |
197 |
97.5 |
| |
|
C. chiopterus |
7 |
72 |
63.9 |
- |
- |
197 |
98.9 |
| |
Sample type |
Ground midge |
4 |
112 |
64.3 |
24 |
70.8 |
309 |
90.6 |
| |
|
Whole midge |
5 |
80 |
81.2 |
20 |
75.0 |
198 |
100.0 |
| |
|
DNA sample |
7 |
96 |
86.5 |
20 |
100.0 |
288 |
95.5 |
| |
DNA extraction method |
Crude lysate |
1 |
28 |
39.3 |
6 |
100.0 |
78 |
83.3 |
| |
|
Squish buffer |
1 |
16 |
56.2 |
4 |
100.0 |
27 |
100.0 |
| |
|
Commercial kits |
5 |
244 |
81.5 [77.3-100.0] |
54 |
90.0 [20.0-100.0] |
661 |
95.6 [95.0-100.0] |
| ITS2/ITS1 |
Overall |
1 |
64 |
90.6 |
14 |
100.0 |
177 |
99.4 |
|
| |
Species |
C. obsoletus |
/ |
33 |
97.0 |
|
|
90 |
100.0 |
| |
|
C. scoticus |
/ |
32 |
87.5 |
|
|
94 |
98.9 |
| |
|
C. dewulfi |
/ |
31 |
100.0 |
|
|
92 |
100.0 |
| |
|
C. chiopterus |
/ |
32 |
96.9 |
|
|
92 |
100.0 |
| |
Sample type |
Ground midge |
/ |
56 |
92.9 |
6 |
100.0 |
156 |
100.0 |
| |
|
Whole midge |
/ |
32 |
93.8 |
4 |
100.0 |
93 |
98.9 |
| |
|
DNA sample |
/ |
40 |
100.0 |
4 |
100.0 |
120 |
100.0 |
| Microarray | Overall | 1 | 64 | 100.0 | 14 | 100.0 | 192 | 100.0 | |
Probabilities (expressed as percentages) are written in bold font when the corresponding effect was significantly different from 0 (α = 0.05). Minimum and maximum values are shown in brackets. DNA extraction method is not tested for both ITS2/ITS1 and microarray because one method was used. For the microarray, the number of samples tested is the same as for the ITS2/ITS1.