Table 2.
Percent amino acid composition of yeast PFDs and human disease-associated PrLDs
| Gln/Asn | Ser | Gly | Tyr | Chargeda | Hydrophobicb | |
|---|---|---|---|---|---|---|
| Yeast PFDsc | ||||||
| Ure2 | 48.3 | 11.2 | 5.6 | 0 | 11.2 | 15.7 |
| Rnq1 | 43.1 | 15.4 | 16.7 | 5.9 | 2.4 | 8.3 |
| Sup35 | 45.6 | 3.5 | 16.7 | 17.5 | 4.4 | 3.5 |
| PFD averaged | 45.7 | 10.0 | 13.0 | 7.8 | 6.0 | 9.2 |
| Yeast genome | 10.0 | 9.0 | 5.0 | 3.4 | 24.0 | 28.3 |
| Human PrLDse | ||||||
| TDP-43 | 21.8 | 15.9 | 26.8 | 0.7 | 3.5 | 15.9 |
| FUS | 21.5 | 22.8 | 28.3 | 12.2 | 3.8 | 0.8 |
| TAF15 | 27.7 | 22.4 | 15.1 | 15.1 | 10.6 | 1.3 |
| EWSR1 | 18.9 | 15.4 | 9.6 | 13.6 | 3.6 | 4.0 |
| hnRNPA2B1 | 12.7 | 9.6 | 45.2 | 10.8 | 9.5 | 6.4 |
| hnRNPA1 | 12.3 | 16.0 | 42.2 | 8.0 | 9.7 | 8.1 |
| TIA1 | 31.6 | 4.2 | 15.8 | 9.5 | 2.1 | 12.6 |
| PrLD average | 20.9 | 15.2 | 26.1 | 10.0 | 6.1 | 7.0 |
| Human genome | 8.3 | 8.1 | 6.6 | 2.8 | 22.9 | 26.5 |
| PrPf | 12.8 | 5.7 | 9.9 | 7.8 | 20.6 | 19.9 |
aCharged residues include D, E, K, R
bHydrophobic residues include F, I, L, M, V
cPFDs are as defined in Table 1
dAverage of the Ure2, Sup35, and Rnq1 PFDs
eDisease-associated PrLDs found in RRM-containing proteins, as defined by the Alberti algorithm [21]
fAmino acids 90–230, which constitute the protease-resistant core of prion aggregates