. Author manuscript; available in PMC: 2014 May 18.

Published in final edited form as: Cancer Epidemiol Biomarkers Prev. 2013 Mar 5;22(5):917–926. doi: 10.1158/1055-9965.EPI-12-1211

Table 1.

Associations between personal and pathological characteristics of colorectal cancer cases and the BRAF p.V600E mutation

				All			MMR-proficient			MMR-deficient

		Total	BRAF wildtype (ref)	BRAF mutated	OR(95%CI)^***	p-value	BRAF mutated	OR(95%CI)^***	p-value	BRAF mutated	OR (95%CI)^***	p-value

All		690	637	53			44			9

Gender	Female	343	319	24	ref		18	ref		6	ref
Gender	Male	347	318	29	1.27 (0.71–2.24)^{^}	0.41	26	1.58 (0.84–2.97)^{^}	0.15	3	0.43 (0.11–1.77)	0.24

Age at CRC diagnosis (SD)		46.96 (7.81)	47.06 (7.66)	45.66 (9.41)	0.98 (0.94–1.01)^#	0.18	44.48 (9.21)	0.96 (0.92–0.99)^#	0.02	51.44 (8.59)	1.10 (0.99–1.22)	0.08

Molecular Results

MSI/IHC	MMR proficient	676	632	44	ref
MSI/IHC	MMR deficient (MLH1/PMS2 loss)	14	5	9	38.73 (11.48–130.67)	<0.001

MLH1 methylation	no	36	34	2	ref		0			2	ref
	yes	12	5	7	27.42 (3.18–236.08)	0.003	0			7	27.42 (3.18–236.08)	0.003
	untested	642	598	44			44			0

Pathology

Tumor location	rectum	238	224	14	ref		13	ref		1	ref
	left-sided colon^*	274	254	20	1.27 (0.63–2.59)	0.50	18	1.25 (0.60–2.62)	0.55	2	1.79 (0.16–19.95)	0.64
	right-sided colon^**	169	151	18	1.98 (0.95–4.13)	0.07	12	1.46 (0.64–3.30)	0.37	6	8.50 (1.00–72.14)	0.05
	unknown	9	8	1			1			0

	left-sided/rectum	512	478	34	ref		31	ref		3	ref
	right-sided	169	151	18	1.73 (0.94–3.17)	0.08	12	1.26 (0.64–2.58)	0.48	6	6.01 (1.46–24.71)	0.01
	unknown	9	8	1			1			0

Grade of Tumor	Low Grade (Well + moderate)	551	522	29	ref		23	ref		6	ref
	High Grade (Poor + undifferentiated)	121	98	23	4.12 (2.28–7.44)	<0.001	20	4.38 (2.30–8.32)	<0.001	3	3.33 (0.79–14.03)	0.10
	unknown	18	17	1			1			0

Mucinous	no	628	582	46	ref		39	ref		7	ref
	yes	50	44	6	1.76 (0.71–4.37)	0.22	4	1.38 (0.47–4.09)	0.56	2	3.44 (0.68–17.42)	0.14
	unknown	12	11	1			1			0

Tumor Margin	pushing	372	350	22	ref		15	ref		7	ref
	infiltrating	235	209	26	1.99 (1.09–3.62)	0.02	25	2.86 (1.46–5.61)	0.002	1	0.24 (0.03–1.97)	0.18
	unknown	83	78	5			4			1

Peritumoral lymphocytes	no	359	337	22	ref		21	ref		1	ref
	yes	270	244	26	1.68 (0.93–3.05)	0.09	19	1.30 (0.68–2.50)	0.43	7	7.78 (0.93–64.97)	0.06
	unknown	61	56	5			4			1

Crohn-like Lymphocytes	no	519	481	38	ref		35	ref		3	ref
	yes	105	95	10	1.33 (0.64–2.76)	0.45	5	0.70 (0.26–1.83)	0.46	5	9.46 (2.15–41.66)	0.003
	unknown	66	61	5			4			1

Tumor infiltrating lymphocytes	no	552	516	36	ref		35	ref		1	ref
	mild	93	82	11	1.89 (0.92–3.87)	0.003^{^^}	7	1.18 (0.50–2.77)	0.81	4	26.70 (2.91–244.59)	<0.001 ^{^^}
	marked	15	11	4	5.20 (1.57–17.24)		0			4	230.89 (22.11–2411.36)
	unknown	30	28	2			2			0

Synchronous CRC	no	624	574	50	ref		41	ref		9
	yes	9	8	1	1.26 (0.15–10.38)	0.83	1	1.39 (0.17–11.60)	0.76	0
	unknown	57	55	2			2			0

left colon included splenic flexure, descending colon, sigmoid colon and rectosigmoid junction

^**

right colon included caecum through transverse colon

^***

adjusted for sex and age at diagnosis

^{^}

adjusted for age at diagnosis

adjusted for sex, OR per year of age

^{^^}

p-trend

All ORs were adjusted for age and sex, except the odds ratios for age at diagnosis of colorectal cancer and sex

Right colon = from ileo-caecal junction to splenic flexure

Left colon = descending colon and sigmoid colon

-OR could not be retrieved due to low number (due to missing values)