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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: J Immunol. 2014 Jan 22;192(4):1361–1371. doi: 10.4049/jimmunol.1301809

Figure 1. Dectin-2 Blocking Antibody Inhibits Df-elicited Pulmonary Inflammation in Sensitized WT Mice.

Figure 1

WT mice were sensitized with 1 μg intranasal Df or saline control on days 0 and 1, challenged with 1 μg intranasal Df on days 14, and 15, treated with 200 μg Dectin-2 antibody or a rat IgG2a control i.p. on days 13 and 15, and killed on day 17. A. Experimental diagram. B. Cells from the BAL fluid were counted, cytospin preparations were stained, and 200 cells/slide were counted for specific cell types. Results are means ± SEM (n = 10-12 mice per group) combined from 3 independent experiments. C and D. Pulmonary mononuclear cells were isolated, stimulated with 50 ng/ml PMA and 1 μM ionomycin for 6 h in the presence of 2.5 μM monensin, stained for cell surface expression of CD4 and CD8, and for intracellular expression of IL-4, IL-5, IL-17A, and IFN-γ, and analyzed by flow cytometry. C. Representative dot plots gating on cell size, CD4 and CD8 expression, and IL-17A are shown. D. The total numbers of cells and subsets recruited to the lung are shown. Data are means ± SEM (n = 6-8 mice per group) combined from 2 independent experiments.