Figure 2. Dectin-2 Blocking Antibody Reduces Lymph Node Cytokine Production in Sensitized WT mice and Has No Effect on Serum Immunoglobulins.
WT mice were sensitized, challenged, and treated with antibody as in Fig. 1. A. Peribronchial lymph node cells were isolated, counted, and restimulated for 72 h with 0 or 5 μg/ml Df, and cytokines in the supernatant were measured by ELISA. Data are means ± SEM (n = 10-12 mice per group) combined from 3 independent experiments. B. Serum IgE and serum Df-specific IgG1 were measured by ELISA. Data are means ± SEM (n = 10-12 mice per group) combined from 3 independent experiments.