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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Clin Immunol. 2014 Mar 20;152(0):115–126. doi: 10.1016/j.clim.2014.03.005

Figure 3. Neuroantigen-specific CD8+ Tregs are terminally differentiated.

Figure 3

Bulk CD8+ T cells were isolated from healthy controls and sorted into (A) CD27+/−, (B) CD28+/−, (C) CD45RO+/−, (D) CD62L+/− and (E) NKG2D+/− CD8+ T cell fractions using magnetic bead-based positive selection kits. Individual CD8+ T cell fractions along with bulk CD8+ T cells were used in suppression assays with autologous APCs and CFSE-stained CD4+CD25− T cells. (F) Positively enriched CD8+ T cells were collected and successively enriched for CD27, CD45RO and CD28 respectively. Individual fractions were used in separate suppression assays with autologous APCs and responders. *p < 0.05; Four or more independent experiments were performed with PBMCs from 4 healthy control donors.