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. Author manuscript; available in PMC: 2015 Jan 24.
Published in final edited form as: Chembiochem. 2014 Jan 7;15(2):233–242. doi: 10.1002/cbic.201300687

Figure 4.

Figure 4

Bioactive signals immobilized through HyPER retain their activity. A–F) mMSCs are seeded on the surface of Q-RGD modified surfaces using the conditions indicated underneath each image. B) Only when light exposure is combined with Q-RGD and FXIIIa do cells spread as well as when E) RGD is introduced to the bulk of the hydrogel. G) RGD patterned in 100μm circles, H) cells cluster to the location of the pattern, I) overlay of the pattern and cell cluster. J–M) HUVECs are cultured on the surface of Q-VEGF modified hydrogels. Hydrogels were treated with decreasing amounts of light to result in 80% to 0% deprotection and QVEGF was immobilized using HyPER. J) 80% and K) 40% deprotection resulted in more cells at the cell surface than L) 10% and M) 0% deprotection. O) HUVEC proliferation was quantified. Statistical analysis was performed using an ANOVA with a Tukey post-test. * and ** indicate that that condition is significantly different from every other condition by at least p < 0.05 or 0.01, respectively.