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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Protein Expr Purif. 2014 Mar 6;98:46–62. doi: 10.1016/j.pep.2014.02.015

Figure 1. Generalized PDZ Domain Affinity Chromatography Purification Scheme I: PDZ Domain Peptide Ligand Tag.

Figure 1

An expressed Protein Of Interest (POI), containing a naturally occurring or recombinantly appended PDZ Domain Peptide Ligand, can be captured from a cellular lysate by binding to a PDZ Domain coupled to a solid support resin. Extensive washing of the captured POI removes cellular contaminants. Free PDZ domain peptide ligand is then added to competitively elute the captured POI.