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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Protein Expr Purif. 2014 Mar 6;98:46–62. doi: 10.1016/j.pep.2014.02.015

Figure 10. Functional Analysis of Purified LacZ Fusion Proteins.

Figure 10

Michaelis-Menten plots for purified LacZ fused to (A) C-terminal PDZ2 domain peptide ligand; and (B) N-terminal PDZ2 domain. β-Galactosidase activity of LacZ was assayed by measuring hydrolysis of 0-2 mM ONPG for 10 minutes at 20 °C. Michaelis-Menten constants (Table 4), were calculated as described in Methods.