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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Protein Expr Purif. 2014 Mar 6;98:46–62. doi: 10.1016/j.pep.2014.02.015

Figure 6. PDZ Domain Peptide Ligand Affinity Tags: Fusion of GluN2B Peptide Ligand Truncation Library to GST.

Figure 6

All samples and Precision Plus Protein All Blue Standards were fractionated on a 4-12% gradient SDS-PAGE gel and stained with Gel Code Blue.

(A) Soluble lysate after heterologous expression of Glutathione S-Transferase (GST) fused to a truncation library of the C-terminal ten residues of the GluN2B Tail.

(B) Glutathione S-Transferase (GST) fusion proteins shown in (A), after purification on a PDZ2 Domain-HaloTag-HaloLink resin and elution with 400 μg/ml SIESDV peptide.