Table 3. Functional Analysis of DasherGFP and Purified DasherGFP Fusion Proteins.

Fluorescence emission at 520 nm, relative to untagged DasherGFP, of equimolar amounts of purified DasherGFP fused to a C-terminal PDZ2 Domain Peptide Ligand, an N-terminal PDZ2 Domain, and single or tandem PDZbh Domains.

Affinity Tag	Relative Fluorescence at 520 nm (%)
Untagged DasherGFP	100
C-terminal PDZ Domain Peptide Ligand	99.2
N-terminal PDZ Domain	97.5
N-terminal 1x PDZ Domain+Beta Hairpin	90.1
N-terminal 2x PDZ Domain+Beta Hairpin	0.3