. Author manuscript; available in PMC: 2015 Jun 1.

Published in final edited form as: Protein Expr Purif. 2014 Mar 6;98:46–62. doi: 10.1016/j.pep.2014.02.015

Table 4. Michaelis-Menten Constants of LacZ and Purified LacZ Fusion Proteins.

Comparison of k_cat and K_M values for LacZ and purified LacZ fused to a C-terminal PDZ2 Domain Peptide Ligand or an N-terminal PDZ2 Domain.

Affinity Tag	k_cat (sec^-1)	K_M (μM)
None	345^*	161^*
C-terminal PDZ Domain Peptide Ligand	413±38	136±44
N-terminal PDZ Domain	390±10	227±21

Wallenfels K. and Malhotra P. (1962) Advanced Carbohydrate Chemistry: 239-298