. Author manuscript; available in PMC: 2015 Jun 1.

Published in final edited form as: Protein Expr Purif. 2014 Mar 6;98:46–62. doi: 10.1016/j.pep.2014.02.015

Table 5. Michaelis-Menten constants of CAT and Purified CAT fusion proteins.

Comparison of k_cat and K_M values for CAT and purified CAT fused to a C-terminal PDZ2 Domain Peptide Ligand or an N-terminal PDZ2 Domain.

	Chloramphenicol^**		Acetyl-CoA^***
Affinity Tag	k_cat (sec^-1)	K_M (μM)	k_cat (sec^-1)	K_M (μM)
None^*	0.43 ± 0.11	80 ± 40	0.085 ± 0.004	41 ± 7
C-terminal PDZ Domain Peptide Ligand	0.37 ± 0.03	8 ± 2	0.074 ± 0.02	120 ± 50
N-terminal PDZ Domain	0.37 ± 0.06	26 ± 10	0.093 ± 0.008	78 ± 20

CAT was purchased from Sigma Aldrich (Cat. No. C8413, St. Louis MO).

^**

Chloramphenicol concentration ranged from 0-100 μM. Acetyl-CoA concentration was fixed at 500 μM.

^***

Acetyl-CoA concentration ranged from 0-500 μM. Chloramphenicol concentration was fixed at 100 μM.