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. 2014 Jun;20(6):882–898. doi: 10.1261/rna.041822.113

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© 2014 Wu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — CPS operon. (A) The genes in operon 108, from SSU0515 to SSU0523, for the biosynthesis of CPS. Reads from the control condition (PC+/−), pig blood (PB+/−), and pig CSF (PCSF+/−) libraries mapped onto CPS operon annotations in positive and negative strands (genome position at the bottom). Scales (0–200) give normalized depths (the number of mapped clean reads for each gene was counted and then normalized to the RPKM value). (B) Dot-blot evaluating the amount of CPS using anti-serotype 2 polyclonal antibody absorbed by P1/7 CPS-deficient mutant. Dilutions of cells were made in PBS and spotted onto nitrocellulose membranes (1 = 10⁶ CFU); the cells were then fixed and the membranes were probed with the absorbed antibody. A P1/7 CPS-deficient mutant generated by transposon mutagenesis was used as a control.