Fig. 2.

VEGF isoforms are absent in VKO mice at P45. Total liver RNA was isolated from P45 control and VKO mice and synthesized to cDNA. A semiquantitative reverse transcription PCR was performed to analyze loss of VEGF isoforms in VKO mice. A primer set that amplifies VEGF isoforms 120, 164, and 188 was used to assess VEGF (A), and GAPDH (B) was used to demonstrate cDNA concentrations and quality. In the control samples, all three VEGF isoforms amplified with isoform 164 being most predominant. All three VKO mice tested did not show any amplification of any of the VEGF isoforms, indicating a sufficient loss of VEGF as anticipated. Five amplification cycles were analyzed (25, 30, 35, 40, and 45). L corresponds to a 100-bp DNA ladder.