Table 1.
Antibodies and reagents used for immunostaining
| Retrieval | Dilution | Host | Company | Amplification | |
|---|---|---|---|---|---|
| Primary antibodies used for IHC | |||||
| Antigen | |||||
| Cytokeratin 19 (TROMA III) | Sodium citrate | 1:50 | Rat | DSHB | Vector Blue |
| CPS1 | Sodium citrate | 1:500 | Rabbit | Abcam | |
| DBA-biotin | Sodium citrate | 1:500 | Vector | ABC; DAB | |
| Endomucin (paraffin) | Sodium citrate | 1:200 | Goat | R&D | ABC; DAB |
| Endomucin (frozen) | 1:200 | Goat | R&D | ||
| Glutamine synthetase | Sodium citrate | 1:1,000 | Mouse | BD Transduction | Vector Blue |
| Hypoxyprobe | Sodium citrate | 1:100 | Mouse | NPI | Vector Blue |
| IsolectinB4-biotin | Sodium citrate | 1:100 | Sigma | ABC; DAB | |
| Laminin | Tris pH 10 | 1:200 | Rabbit | BioGenex | ABC; DAB |
| PECAM (frozen) | 1:100 | Rat | BD Pharmingen | ||
| Smooth muscle actin | Sodium citrate | 1:1,000 | Mouse | Sigma | ABC; DAB |
| Secondary antibodies used for IHC | |||||
| α−Rabbit-biotin | 1:500 | Donkey | Jackson ImmunoResearch | ||
| α-Rabbit-cy3 | 1:300 | Donkey | Jackson ImmunoResearch | ||
| α-Rat-Alexa488 | 1:300 | Donkey | Jackson ImmunoResearch | ||
| α-Rat-alkaline phosphatase | 1:500 | Donkey | Jackson ImmunoResearch | ||
| α-Goat-biotin | 1:500 | Donkey | Jackson ImmunoResearch | ||
| α-Mouse-biotin | 1:500 | Goat | Jackson ImmunoResearch | ||
| α-Mouse-alkaline phosphatase | 1:500 | Donkey | Jackson ImmunoResearch | ||
| Streptavidin-cy2 | 1:300 | Jackson ImmunoResearch | |||
| Reagents | |||||
| ABC | Vector | ||||
| Vector Blue | Vector | ||||
| DAB | Vector | ||||
IHC, immunohistochemistry; CPS1, carbamoyl phosphate synthetase 1; DBA, Dolichos biflorus agglutinin; ABC, avidin-biotin complex; DAB, 3,3′-diaminobenzidine; PECAM, platelet endothelial cell adhesion molecule.