Fig. 8.

Labeled CoRL acquire ultrastructural features of podocytes. To assess ultrastructural features labeled CoRL, immunoperoxidase staining for ZsGreen reporter was performed. A: image of immunoperoxidase staining for the ZsGreen reporter (brown) in the vascular smooth muscle compartment (indicated by arrow, positive control). B: immunoperoxidase staining for the ZsGreen reporter (black) on ultrathin section viewed by transmission electron microscopy (TEM) was detected in the vascular smooth muscle compartment, used as a positive control (arrows indicate examples). C: higher magnification image of reporter staining in the area indicated by the dashed square shown in B. D: immunoperoxidase staining for reporter (brown) in the intraglomerular compartment appears in a podocyte distribution (indicated by arrow). E: TEM image of podocyte with reporter staining (black) in both the cell body and within foot processes. F: higher magnification image of the area indicated by the dashed square shown in E. Arrows indicate staining in foot processes. G: even higher magnification image of the area indicated by the dashed square shown in F. Arrow indicates reporter staining in foot processes. H: lower magnification image of podocyte that lacked reporter staining in the cell body or within foot processes. I: higher magnification image of reporter staining (black) in the area indicated by the dashed square shown in H. Arrows indicate the lack of staining in foot processes. J: higher magnification image of the area indicated by the dashed square shown in I. Arrows indicate the lack of staining in foot processes.