Table 1.
Morphometric analysis of the kidney under light microscopy
| SS Rats |
SS.13BN Rats |
SD Rats |
||||
|---|---|---|---|---|---|---|
| 0.4% NaCl diet | 4% NaCl diet | 0.4% NaCl diet | 4% NaCl diet | 0.4% NaCl diet | 4% NaCl diet | |
| Glomeruli, number of glomeruli/mm2 cortex | 3.7 ± 0.2 | 3.5 ± 0.1 | 3.6 ± 0.3 | 3.8 ± 0.2 | 3.4 ± 0.1 | 4.0 ± 0.2 |
| PCT diameter, μm | ||||||
| Superficial | 38.0 ± 0.6 | 38.2 ± 0.1 | 38.0 ± 0.6 | 36.5 ± 0.8 | 40.7 ± 0.8 | 40.4 ± 1.1 |
| Juxtamedullary | 38.7 ± 0.4 | 39.1 ± 0.6 | 39.0 ± 0.5 | 38.0 ± 0.8 | 38.6 ± 0.5 | 36.6 ± 1.1 |
| PCT cell height, μm | ||||||
| Superficial | 11.7 ± 0.3 | 11.3 ± 0.2 | 11.3 ± 0.2 | 10.9 ± 0.3 | 11.7 ± 0.4 | 11.4 ± 0.2 |
| Juxtamedullary | 11.8 ± 0.3 | 11.2 ± 0.2 | 11.5 ± 0.1 | 11.5 ± 0.2 | 12.1 ± 0.1 | 10.6 ± 0.4 |
| DT diameter, μm | 26.2 ± 0.3 | 29.2 ± 0.1* | 26.4 ± 0.2 | 25.9 ± 0.4 | 26.8 ± 0.1 | 26.8 ± 0.1 |
| DT cell height, μm | 8.9 ± 0.2 | 8.6 ± 0.1 | 8.8 ± 0.1 | 8.8 ± 0.1 | 8.7 ± 0.3 | 8.6 ± 0.2 |
| DT cell number, number of cells/DCT | 9.6 ± 0.2 | 10.0 ± 0.2 | 9.5 ± 0.2 | 9.9 ± 0.2 | 9.6 ± 0.4 | 9.6 ± 0.2 |
| mTAL diameter, μm | ||||||
| Outer stripe | 26.1 ± 0.2 | 27.2 ± 0.5 | 26.7 ± 0.4 | 27.2 ± 0.5 | 25.9 ± 0.6 | 28.9 ± 0.9 |
| Inner stripe | 26.2 ± 0.4 | 26.7 ± 0.8 | 25.5 ± 0.4 | 24.7 ± 0.5 | 25.1 ± 0.2 | 25.8 ± 0.4 |
| mTAL cell height, μm | ||||||
| Outer stripe | 8.8 ± 0.2 | 8.9 ± 0.1 | 9.3 ± 0.2 | 8.9 ± 0.2 | 8.5 ± 0.2 | 9.2 ± 0.2 |
| Inner stripe | 7.9 ± 0.3 | 8.1 ± 0.2 | 8.1 ± 0.3 | 8.0 ± 0.1 | 7.6 ± 0.1 | 8.6 ± 0.2 |
| Interlobular artery | ||||||
| Lumen/vessel | 0.20 ± 0.02 | 0.19 ± 0.02 | 0.19 ± 0.02 | 0.19 ± 0.01 | 0.22 ± 0.01 | 0.19 ± 0.01 |
| Wall/vessel | 0.80 ± 0.02 | 0.81 ± 0.02 | 0.81 ± 0.02 | 0.81 ± 0.01 | 0.79 ± 0.01 | 0.81 ± 0.01 |
Values are means ± SE; n = 4–6 rats/group.
PCT, proximal convoluted tubule; DT, distal tubule; mTAL, medullary thick ascending limb.
*
P < 0.05 vs. Dahl salt-sensitive (SS) rats on a 0.4% NaCl diet and SS.13BN and Sprague-Dawley (SD) rats on a 4% NaCl diet.