Skip to main content
. 2014 May 12;5:3837. doi: 10.1038/ncomms4837

Figure 3. Lower abundance of complex I matrix subunits in long-living mice.

Figure 3

All data are mean±s.e.m. from four animals per group, *P<0.05, **P<0.01 (t-test). (a) SDS–PAGE western blots of complex I matrix arm subunits NDUFS3 and NDUFV2, PhB and membrane arm subunits NDUFA9 and NDUFB9 in liver mitochondria. Each sample is from a different animal. (b) Relative abundance of matrix subunits NDUFS3 and NDUFV2 and PhB to membrane subunits NDUFA9 and NDUFB9 in liver mitochondria from 7–8-month-old C57Bl/6 mice under AL (open bars) and DR (filled bars). (c) As in b, comparing long-lived ICRFa mice (filled bars) with control C57Bl/6 (open bars) at 7–8 months of age. (d) As in b, comparing young (filled bars) to old (open bars) ICRFa liver mitochondria. (e) SDS–PAGE western blots of the indicated proteins in brain mitochondria. (f) Relative abundance of matrix subunits NDUFS3 and NDUFV2 and PhB to the membrane subunit NDUFA9 in brain mitochondria from 15-month-old C57Bl/6 mice under AL (open bars) and DR (started at 3 months of age, filled bars). (g) SDS–PAGE western blots of the indicated complex I subunits in skeletal muscle mitochondria. (h) Relative abundance of matrix subunit NDUFV2 to the membrane subunit NDUFB9 in muscle mitochondria from 15-month-old C57Bl/6 mice under AL (open bar) and DR (filled bar).