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Confocal micrographs showing 21-day EaRASMC layers cultured with blank (active agent controls) and HA-o-loaded PVA-NPs. The cultures were immunofluorescence- labeled for elastin (false-colored magenta; white arrows), which is mostly localized extracellularly. Nuclei were stained with DAPI (blue) and filamentous actin was stained with Alexa Fluor 488 phalloidin (green).
