FIG. 5.

PD-L1 upregulation in PCa cells after treatment with recombinant human IFN-γ is not abrogated by radiation. (A) DU145 cells and (B) PC3 cells were treated with 10 ng IFN-γ (hatched bar) or left untreated (open bar). Surface PDL-1 was analyzed after 72 hours. Numbers indicate percent-positive cells. *Statistical significance relative to untreated cells. (C) DU145 cells and (D) PC3 cells were irradiated (10 Gy, gray bar), treated with 10 ng IFN-γ (hatched bar) or first irradiated, then treated with IFN-γ after 24 hours (hatched gray bar); untreated (open bar). Surface PDL-1 was analyzed after 72 hours. Numbers indicate fold change normalized to untreated cells.