Fig. 1.
Schematic representations of the dsAegyptin and 30KExGM transgenes. (A) The plasmid containing the transgene dsAegyptin is engineered to express an anti-Aegyptin dsRNA under the control of the 30K bidirectional promoter. 30K a drives the expression of the reporter gene, eGPF, and 30K b, the Aegyptin promoter, drives the Aegyptin-specific dsRNA. The transformation marker gene DsRED is under control of the 3XP3 eye-specific promoter (11). (B) The transgene inserted in the control transgenic line 30KExGM has a similar organization and was engineered to express Mnp, an antidengue dsRNA. Detailed descriptions of pMos_dsAegyptin and pMos_30KExGM are in Fig. S1 and Mathur et al. (11), respectively. Dashed arrows indicate direction of transcription.