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. 2014 Apr 28;111(19):6946–6951. doi: 10.1073/pnas.1404179111

Fig. 2.

Fig. 2.

Quantification of Aegyptin gene expression in transgenic mosquitoes. (A) Accumulation of Aegyptin mRNA in salivary glands was quantified by using qRT-PCR. Expression of dsRNAi in dsAegyptin mosquitoes reduced significantly the Aegyptin mRNA level relative to the 30KExGM control (P < 0.0001). Biological replicates (n = 3) containing 20 salivary gland pairs each were assayed in duplicate. (B) dsRNA-mediated silencing of Aegyptin transcription products reduces significantly corresponding protein levels in transgenic dsAegyptin mosquitoes. ****P < 0.0001. n.s, not significant.