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. 2014 Apr 28;111(19):E2008–E2017. doi: 10.1073/pnas.1402944111

Fig. 1.

Fig. 1.

Generated cell lines were characterized. (A and B) Expression of RET and GFRα1 mRNA by generated MiaPaCa-2 and 3T3 cell lines. (C) Immunofluorescence microscopy for RET (red), GFRα1 (green), and DAPI (blue) in generated MiaPaCa-2 and 3T3 cell lines. (D) Boyden chamber migration assays using glial cell line-derived neurotrophic factor (GDNF) as an attractant over 24 h for MiaPaCa-2 derived cell lines. (E) MiaPaCa-2 siRNA control, siRNA RET, and siRNA GFRa1 cell proliferation over 24 h. (F) Boyden chamber migration assays using GDNF, GDNF plus soluble GFRα1-Fc fusion protein, or excised murine DRG as an attractant over 24 h for 3T3-derived cell lines. (G) 3T3-RET-GFRα1, 3T3-RET, and 3T3 cell proliferation are similar over 24 h.