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. 2014 Apr 28;111(19):E2008–E2017. doi: 10.1073/pnas.1402944111

Fig. 3.

Fig. 3.

DRG release soluble GFRα1 that promotes cell migration and RET activation. (A) Conditioned media from excised murine DRG following 0, 4, 6, or 8 d of exposure were concentrated and underwent Western blotting to detect released GFRα1. Total protein measured by Ponceau staining was used as a loading control. (B) 3T3-RET cells were cocultured with DRG from 0 to 12 h, and protein was isolated for Western blotting. (C and D) 3T3-RET cells underwent Boyden chamber migration assays over 24 h using conditioned media from DRG as an attractant with the addition of varying concentrations of an anti-GFRα1 antibody; Western blots were performed at parallel conditions. (E and F) MiaPaCa-2 cells underwent Boyden chamber migration assays over 24 h using conditioned media from DRG as an attractant with the addition of varying concentrations of an anti-GFRα1 antibody; Western blots were performed at parallel conditions.