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. 2014 Apr 28;111(19):E2008–E2017. doi: 10.1073/pnas.1402944111

Fig. 4.

Fig. 4.

GFRα1 potentiates PNI in a nerve–cancer coculture in vitro assay. (A) Coculture of murine DRG with MiaPaCa-2 cells in Matrigel permits assessment of the degree of PNI. By day 6, MiaPaCa-2 cells exposed to IgG as a control condition exhibit robust invasion, extending along neurites from the DRG. (B) DRG–MiaPaCa-2 coculture assay of PNI, when exposed to anti-GFRα1 antibody, demonstrates diminished PNI at day 6 compared with control (A). (C) The degree of PNI may be quantified in the DRG–MiaPaCa-2 coculture assay. Areas where MiaPaCa-2 cells are in direct contact with DRG neurites were demarcated, and the area was calculated using MetaMorph software. (D) The mean total area of invasion is compared between control IgG and anti-GFRα1 antibody exposed DRG–MiaPaCa-2 coculture assays (†P < 0.05; t test).