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. 2014 Apr 28;111(19):E2008–E2017. doi: 10.1073/pnas.1402944111

Fig. 8.

Fig. 8.

Histologic images of proximal sciatic nerve PNI by cancer cells lacking GFRα1 maintain an ability to invade nerves. (A) H&E-stained normal proximal sciatic nerve 6 wk after injection with PBS shows normal proximal sciatic nerve histology and caliber. (Scale bar, 500 µm.) (B) H&E-stained proximal sciatic nerve 6 wk after injection with Mia shControl cells demonstrates extensive PNI with expansion of the nerve by infiltrating tumor cells. (Scale bar, 500 µm.) (C) H&E-stained proximal sciatic nerve 7 wk after injection with Mia shGFRα1 cells demonstrates proximal sciatic PNI, although to a lesser severity compared with the shControl cells. (Scale bar, 500 µm.) (D) H&E-stained proximal sciatic nerve 9 wk after injection with Mia shRET cells demonstrates no significant PNI. (Scale bar, 500 µm.) (E) Immunofluorescence microscopy was performed on excised Mia shControl, shRET, and shGFRα1 primary sciatic nerve tumors stained for RET and GFRα1 expression (upper images) and p-RET expression (lower images) with DAPI nuclear staining. (Scale bar, 100 µm.)