Cytokinin content and cytokinin response marker gene expression in shoots and roots of wild-type and atabcg14 plants. (A and B) Cytokinin concentrations in shoots (A) and roots (B). Shoots and roots of plants grown on MGRL-agar medium for 14 d were collected for cytokinin quantification. tZ, iP, cZ, and DZ type represent the concentration of each type of cytokinin present, and the individual cytokinins belonging to each type are described in Fig. S5. (C) Expression levels of type-A ARRs in shoots (Upper) and roots (Lower) of wild-type (WT) and atabcg14 (abcg14) plants. Note that the expression of ARRs is reduced relative to the wild type in abcg14 shoots, but increased in abcg14 roots. Values are mean ± SEM (n = 4 each from two independent experiments). *P < 0.05, **P < 0.01 (Student t test) relative to expression of the wild-type control. (D) TCS::GFP expression pattern in the roots of the wild type and abcg14. (Scale bar, 50 µm.)