Fig. 4.

Neurosteroids modulate the membrane insertion of the α4 subunit in hippocampal neurons. (A) The 10–15 Div hippocampal neurons expressing RFPα4 or RFPα4(S443A) subunits were subject to TIRF for 5 min before (basal) and 5 min after 20-min incubation at 37 °C with 100 nM THDOC. The images in the lower panels are enlargements of the boxed regions in the upper panels. (B) The total number of insertion events per minute was then calculated in the absence and presence of THDOC. *, significantly different from control (t test, P < 0.05; n = 5–7).