Fig. 6.

Lipid abnormalities and chromatin defects in seipin-deficient male germ cells. (A) Electron microscopic analysis reveals large LDs in spermatids from the seminiferous epithelium of S-KO males and much smaller LDs in residual bodies in tubules from S-WT males. Black dotted line outlines the contour of LDs; arrows show residual bodies. (B) In contrast to spermatids from control littermates, round, elongating, and elongated spermatids from S-KO males contain large ectopic LDs and exhibit defective compaction and condensation of nuclear chromatin and abnormal morphology. (C) The seminiferous epithelium of S-KO males contains degenerating clusters of several spermatids and large LDs. Clusters of spermatid tails are visualized as bundles in longitudinal sections. Arrows, LDs; arrowheads, nuclei.