Fig. 3.

Cell cycle-specific cleavage and processing of Scc2. (A) Full-length and cleaved Scc2 forms immunoprecipitated from late S- or M-staged extracts (1891-32C) were subjected to MS. Crude (C), precleared extract (PC), immunoprecipitated (IP), and flow through (FT) samples are shown. (B) Scc2 peptides spanning residues 140–189 identified by MS in tryptic digests from late S phase or mitotic cells are shown. Full tryptic peptides are flanked by periods and the adjacent amino acids. Semitryptic peptides are underlined.