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. 2014 Apr 29;111(19):E1950–E1959. doi: 10.1073/pnas.1406161111

Fig. 6.

Fig. 6.

I287D interacts with R3 in the depolarized state of the VSD. (A) Activation gating current traces are shown for WT (Left) and the I287D mutant (Right) for the indicated test pulse voltages (HP = −120 mV). Note the apparent immobilization of the gating charge during repolarization for the I287D mutant upon more positive test pulses. (B) Deactivation gating current traces shown for the I287D mutant as a function of the repolarizing pulse voltage. (C) Activation ionic current traces for the conductive WT Shaker and the I287D mutant (recorded at a temperature of 14 °C to better resolve pore closure). (D) Deactivation τ–V curves for WT (open diamonds), I287D (full squares), R3K (full triangles), and I287D/R3K (open circles). (E) Histogram showing the TD values for WT Shaker and the indicated mutants.