Table 1.

Primer sequences used in qRT-PCR analysis to determine the level of gene expression for osteogenic differentiation and BMP-2 bioactivity

Gene	Primer
GAPDH	Sense 5′ GTC GGA GTC AAC GGA TTT GG – 3′ Antisense 5′ – GGG TGG AAT CAA TTG GAA CAT – 3′
Runx2	Sense 5′ – AGA TGA TGA CAC TGC CAC CTC TG – 3′ Antisense 5′ – GGG ATG AAA TGC TTG GGA ACT GC – 3′
ALP	Sense 5′ – CCC TTG ACC CCC ACA ATG T – 3′ Antisense 5′ – GTT GTT CCT GTT CAG CTC GTA – 3′

Abbreviations: qRT-PCR, real-time reverse transcriptase polymerase chain reaction; BMP-2, bone morphogenetic protein-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase.