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Probing the immunosuppressive effects of mesenchymal stem cells (MSCs) on CD4 T cells. (A) Individual preparation of CD4 T cells and MSCs, followed by coculturing in a bulk or microwell assay. (B) Representative images of CD4 T cells after activation (imaged with anti-CD25 [green] and anti-CD69 [red] immunocytochemical staining). (C) Representative images of MSCs and CD4 T cells cocultured in 6-well plates (left arrow: CD4 T cell; right arrow: MSC).
