Effect of NaHS (50 μM·kg−1·day−1 ip for 10 days) on basal and phosphorylated levels of PTEN, Akt, and GSK3β in myocardium from WT and Akt2KO mice. A: representative gel blots depicting expression of pPTEN, PTEN, pAkt, Akt, pGSK3β, and GSK3β using respective specific antibodies. GAPDH was used as loading control. B: pPTEN-to-PTEN ratio. C: pAkt-to-Akt ratio. D: pGSK3β-to-GSK3β ratio. Values are expressed as mean ± SE; n = 4 or 5 mice per group. *P < 0.05 vs. WT group, #P < 0.05 vs. Akt2KO group.