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The primer sequences and the genotyping methods for IL-1α and IL-1β polymorphisms.
| LOCUS AND PRIMER SEQUENCES | ANNEALING TEMPERATURE (°C) | PCR AMPLICON SIZE (BP) |
|---|---|---|
| IL-1α+4845 | 56 | 229 |
| F:5'ATGGTTTTAGAAATCATCAAGCCTAGGGCA3' | ||
| R:5'AATGAAAGGAGGGGAGGATGACAGAAATT3' | ||
| IL-1β+3954 | 58 | 194 |
| F: 5' CTCAGGTGTCCTCGAAGAAATCAA 3' | ||
| R: 5' GCTTTTTTGCTGTGAGTCCCG 3' |
